Contact an Account ManagerSensitive and rapid amino acid analysis of peptide hydrolyzates by HPLC of o-phthaldialdehyde derivatives
Summary
Type: HPLC
Application #: 107800
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: excitation 330nm, emission 420nm
Conditions: eluent A: 0.1M Na citrate pH 6.5, eluent B: methanol, gradient from 20 to 45% B in 5min, 5min isocratic 45% B, then gradient to 75% B in 20min
flow rate 1.0ml/min
temperature 22°C
Authors: Burbach JPH
Source: J Chromatogr 237 (1982) 339 - 343
Details
Legend: Fig. 1. Elution profile of OPT derivatives of amino acid standards (80pmol
each), X originates from the o-phthaldialdehyde - mercaptoethanol reagent.